Exercise- and Drug Dose-dependent Metabolic Assay Device Using the Hydrogel-supported Skeletal Muscle Cells

We have prepared contractile skeletal muscle cellular micropatterns supported by the hydrogel to investigate the relationship between exercise or drug administration and development of type 2 diabetes under controlled culture conditions. The muscle cell-patterned gel sheet can be handled easily while sustaining the cellular structure and activity, allowing for the first time to be combined with the various analytical devices on demand. In this study, two types of bioassay devices were constructed by combining the gel sheet with our original microelectrode-printed flexible gel sheet for electrically inducible exercise-dependent metabolic assay, and with a microfluidics for drug screening.